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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene is observed in many tumors, which leads to chromosome instability, and so may possibly lead to tumorigenesis. This protein was identified to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a vital purpose in endorsing mobile-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, have been explained. Two extra splice variants were being documented but detailed nucleotide sequence information and facts will not be nonetheless accessible.

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Blocking peptides are peptides that bind exclusively to the goal antibody and block antibody binding. These peptide ordinarily consists of the epitope identified via the antibody.

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This guidebook outlines more possible optimization of mobile and immunoassay parameters to be sure the best possible results are...

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This tutorial outlines more probable optimization of cellular and immunoassay parameters to guarantee the best possible outcomes are...

Antibodies certain to the blocking get more info peptide no more bind towards the epitope about the concentrate on protein. By evaluating the staining from the blocked antibody compared to the antibody alone, you can see which staining is unique.

Focus on Info Cyclin E belongs to your highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins perform as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which lead for the temporal coordination of each mitotic party. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S changeover. This protein accumulates with the G1-S phase boundary and is particularly degraded as cells progress through S period.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...

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